Lorenzo Pellegrini has been sidelined with a fractured metatarsal, Roma have confirmed.
Roma earned their first away clean sheet by edging Lecce 1-0 on Sunday afternoon, to keep the pressure on the early pace-setters.
Roma are aiming to bounce back from their first defeat of the season when they take on promoted side Lecce on Sunday afternoon.
Paulo Fonseca fell to his first defeat as Roma coach on Wednesday night as Atalanta won 2-0 at Stadio Olimpico.
Roma face one of their toughest tests of the season so far when they host Atalanta on Wednesday night, in the first midweek Serie A match of the campaign.
A last-gasp winner from Edin Dzeko gave Roma their third consecutive win on Sunday, as Bologna were beaten by the Giallorossi.
Roma venture away from Stadio Olimpico for the first time this season when they travel to face Bologna on Sunday afternoon.
Roma Women are looking to make amends for their disappointing start to the season when they take on fellow title challengers Fiorentina on Sunday.
Roma began their Europa League campaign with a 4-0 win over Istanbul Basaksehir on Thursday night, extending their unbeaten run to 13 games.
Chris Smalling insists he has not taken the easy route by trading Manchester United for Roma.